2023 Publications

Highlights
* = co-first author, † = corresponding author

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Cryo-EM structures of the active NLRP3 inflammasome disk

Xiao L*, Magupalli VG, Wu H † Nature (2022) , 613 (7944) 595-600.. PDF

Inflammasomes are cytosolic innate immune complexes that activate caspase-1 upon detection of pathogenic and endogenous dangers1-5, and NLRP3 is an inflammasome sensor of membrane damage highly important in inducing inflammation2,6,7. Here we report cryo-EM structures of disk-shaped active NLRP3 oligomers in complex with ATP𝛾S, the centrosomal kinase NEK7, and the adaptor protein ASC which recruits caspase-1. In these NLRP3-NEK7-ASC complexes, the central NACHT domain of NLRP3 assumes an ATP-bound conformation in which two of its subdomains rotate by ~85 ° relative to the ADP-bound inactive conformation8-12. ... Read More

Other Publications (2023)

* = co-first author, † = corresponding author

David L*, Martinez L, Xi Q, Kooshesh KA, Zhang Y, Shah JV, Maas RL†, Wu H.† Piezo mechanosensory channels regulate centrosome integrity and mitotic entry. Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2213846120. doi: 10.1073/pnas.2213846120

Paidimuddala B, Cao J, Nash G, Xie Q, Wu H, Zhang L.. Mechanism of NAIP-NLRC4 inflammasome activation revealed by cryo-EM structure of unliganded NAIP5 . Nat Struct Mol Biol. 2023 Jan 5. doi: 10.1038/s41594-022-00889-2.

Du T, Song Y, Ray A, Wan X, Yao Y, Samur MK, Shen C, Penailillo J, Sewastianik T, Tai YT, Fulciniti M, Munshi NC, Wu H, Carrasco RD, Ubiquitin receptor PSMD4/Rpn10 is a novel therapeutic target in multiple myeloma Chauhan D, Anderson KC. Blood. 2023 Jan 11:blood.2022017897. doi: 10.1182/blood.2022017897.

Du G *, Zhao L, Zheng Y, Belfetmi A, Cai T, Xu B, Heyninck K, Van Den Heede K, Buyse MA, Fontana P, Bowman M, Lin LL, Wu H†, Chou JJ† Autoinhibitory structure of preligand association state implicates a new strategy to attain effective DR5 receptor activation.. Cell Res. 2023 Feb;33(2):131-146

Baris S, Benamar M, Chen Q, Catak MC, Martínez-Blanco M, Wang M, Fong J, Massaad MJ, Sefer AP, Kara A, Babayeva R, Eltan SB, Yucelten AD, Bozkurtlar E, Cinel L, Karakoc-Aydiner E, Zheng Y, Wu H, Ozen A, Schmitz-Abe K, Chatila TA. Severe Allergic Dysregulation Due to A Gain of Function Mutation in the Transcription Factor STAT6.. J Intern Med. 2023 Mar;293(3):340-353.

Li J*, Wang L*, Hahn Q, Nowak R , Viennet T, Orellana E, Burman S, Yue H , Hunkeler M, Fontana P, Wu H, Arthanari H, Fischer E, Gregory R. . Structural basis of regulated m7G tRNA modification by METTL1-WDR4. Nature. 2023 Jan 4th, doi 10 1038/s41586- 022005566-4